Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVT--AKIAEVEEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGV-NAREIEVGLLGNYDVKSTLPGEVV-----KDVAFYDYDAKYIDN-KVTMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDLAKESFDKREAHLI 1EHI Chain:A ((135-362)) -----------------------------------------------------------------------------------------------------------------------------DKALTKELLTVNGIRNTKYIVVDPESANNWS--WDKIVAELGNIVFVKAANQGSSVGISRVTNAEEYTEALSDSFQYDYKVLIEEAVNGARELEVGVIGNDQPLVSEIGAHTVPNQGSGDGWYDYNNKFVDNSAVHFQIPAQLSPEVTKEVKQMALDAYKVLNLRGEARMDFLLDENNVPYLGEPNTLPGFTNMSLFKRLWDYSDINNAKLVDMLIDYGFEDFAQNKKLS-

General information: TITO was launched using: RESULT: Template: 1EHI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1029 56128 54.55 256.29 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 54.55 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_1EHI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EHI-query.scw PDB file : Tito_Scwrl_1EHI.pdb: