Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQVDIAALSPQVVWQHFQTLCTIPRPSK------HEQQLREFLQNWAESRNLETYVD--------------EVGNLIIRKNATPGKKHVSGVILQGHLDMVTQANTGTVHDFFKDPIRPVLEEGWLIAKDTTLGADNGIGVALALAVLDSND-----IHGPIEVLLTVDEEAGMSGARLLETGV-----LKGKWLFNIDTEEW----GELYLGCAGSIDVEVEQPLNYEPIPENLNIVN--IQVAGLKGGHSGVDIHLGRGNANVILARFLNQHLASLGGHLVEFTGGTARNALPREAVATI--AI-------SP-NQ-LSSLEKLLAEY-QAAWKEQLKGIDDNLQLSIQSTGAKVTEVINQQQQNEWLQALATSPYGVASMSQVLPDVVETSNNIGVVRLNREGGKAILMVRSMVNQEAQDFAEKIQAHFSQF------NIGSTLT--PLVSGWTPNPDSAALKCLQQAYQNAFNIEPNLKVIHAGLECGI-IAEHYPHLQMVSFGPDI--QGAHAPGERVKVDTVEKCWKLLVTALASVE 2ZOG Chain:A ((16-474)) -------DENQDRYVKKLAEWVAIQSVSAWPEKRGEIRRMMEVAAADVQRLGGSVELVDIGKQKLPDGSEIPLPPILLGKLGSD---PQKKTVCIYGHLDVQPAAL---EDGWDSEPFTLVEREGKLYGRGS---TDDKGPVAGWMNALEAYQKTGQEIPVNLRFCLEGMEESGSEGLDELIFAQKDKFFKDVDYVCISDNYWLGKNKPCITYGLR------------------GICYFFIEVECSDK-DLHSGVY-GGSVHEAMTDLISLMGCLVDK-KGKI-LIPGINDAVAPVTDEEHALYDHIDFDMEEFAKDVGAETLLHSCKKDILMHRWR------YPSLSL--HG-----------------------IEGA-----------FSGSGAKTVIP---RKVVGKFSIRLVPDMIPEVVSEQVSSYLSKKFAELQSPNKFKVYMGHGGKPWVSDFNHPHYQAGRRALKTVFGVEPDLTREGGSIPVTLTFQEAT-GKNVMLLPVGSADDGAHSQNEKLNRLNYIEGTKMLAAYLYEV-

General information: TITO was launched using: RESULT: Template: 2ZOG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1798 -3207 -1.78 -8.02 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -1.78 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_2ZOG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZOG-query.scw PDB file : Tito_Scwrl_2ZOG.pdb: