Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYHGME-----QPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKE-KNVWVVSQNIDGLHAKAKS--PQLVNFHGSLYQCHCRKCQQTVDWKEYL------HSDKHQAC--------------------GGQIRPDIVLYGEGFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEPYTFIQEDGAVVFKKIQELGADYD 2HJH Chain:B ((33-316)) --FFTIDHFIQKLHTARKILVLTGAGVSTSLGIPDFRSSEGFYSKIKHLGLDDPQDVFNYNIFMHDPSVFYNIANMVLPPEKIYSPLHSFIKMLQMKGKLLRNYTQNIDNLESYAGISTDKLVQCHGSFATATCVTCHWNLPGERIFNKIRNLELPLCPYCYKKRREYFSMSERPPYILNSYGVLKPDITFFGEALPNKFHKSIREDILECDLLICIGTSLKVAPVSEIVNMVPSHVPQVLINRDPVKHAEFDLSLLGYCDDIAAMVAQKC----

General information: TITO was launched using: RESULT: Template: 2HJH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1100 -42200 -38.36 -179.57 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -38.36 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_2HJH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HJH-query.scw PDB file : Tito_Scwrl_2HJH.pdb: