Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQEQIEKKKEEKQKAEKAALEKK 1ETF Chain:B ((1-23)) -------------------------------------DTRQARRNRRRRWRERQRAAAAR----

General information: TITO was launched using: RESULT: Template: 1ETF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 16 2604 162.75 113.22 target 2D structure prediction score : 0.87 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 162.75 2D Compatibility (Sec. Struct. Predict.) : 0.87 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 1.001

(partial model without unconserved sides chains): PDB file : Tito_1ETF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ETF-query.scw PDB file : Tito_Scwrl_1ETF.pdb: