Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKPIIAGNWKMNKTASEAKEFAEAVKN-KIPSNDKVDSVIGSPALFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYFHETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVSNMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPENIAEYMAKENVDGALVGGASLQADSFLSLLDAVK 4FF7 Chain:B ((3-246)) -RTFFVGGNFKLNGSKQSIKEIVERLNTASIPEN--VEVVICPPATYLDYSVSLVKKPQVTVGAQNAYLKASGAFTGENSVDQIKDVGAKWVILGHSERRSYFHEDDKFIADKTKFALGQGVGVILSIGETLEEKKAGKTLDVVERQLNAVLEEV--KDWTNVVVAYEPVWAIGTGLAATPEDAQDIHASIRKFLASKLGDKAASELRILYGGSANGSNAVTFKDKADVDGFLVGGASLKPE-FVDIINS--

General information: TITO was launched using: RESULT: Template: 4FF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1329 1390 1.05 5.72 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : 1.05 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_4FF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FF7-query.scw PDB file : Tito_Scwrl_4FF7.pdb: