Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILEIMMKVKNIQSETAMKQHSESVVSNQFEPTCHSEHSSVSDVCSSSAFGLATRSSRARMKRASGFTLIEVMVVIVILGVLAALIVPNVMGRSEKAKIDTTQITLKGVAGALDQYKLDNGHFPTMQEGGLDALINQPA---TAKNWMPGGYVKGGYPKDSWKNDIQYVVPGKDDHPFDLYSFGADGKEGGEGNDADIYYQP 3G20 Chain:B ((5-116)) -----------------------------------------------------------------------------------SLVVPNLMGNKDKADRQKVMSDLVALESTLDMYRLDNNRYPTTE-QGLRALVSKPTVQPEPRNYRQDGYIRR-LPQDPWGGDYQLLNPGQY-SDIDIFSPGPDGVPNTED---DIGNW-

General information: TITO was launched using: RESULT: Template: 3G20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 400 20161 50.40 184.96 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 50.40 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.617

(partial model without unconserved sides chains): PDB file : Tito_3G20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G20-query.scw PDB file : Tito_Scwrl_3G20.pdb: