Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILTITMNPSIDSIYFTKHFE-LGTMTRFDSPVRYVGGKGINCGRASALLGSSVILTGFLGGY-FGSMIQDMLKNENLFDLHFLPIA-EESRGAVTIMHDDET-Q-TELVEAGPEIADTTIKKLMNDI-KLISKNNYLSVITINGSVNNP-DPFLYAKLLQYIKEQIDPSTYILMDISGQHLRE-VLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPLFKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPLKECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK 3Q1Y Chain:A ((3-309)) LIYTITLNPAIDRLLFIRGELEKRKTNRVIKTEFDCGGKGLHVSGVLSKFGIKNEALGIAGSDNLD-KLYAILKEKH-INHDFLVEAGTSTRECFVVLSDD-TNGSTMIPEAGFTVSQTNKDNLLKQIAKKVKK---EDMVVIAGSPPPHYTLSDFKELLRTVK---ATGAFLGCDNSGEYLNLAVE---MGVDFIKPNEDEVIAILDEKTN---SLEENIRTLAE--KIPYLVVSLGAKGSICAHNGKLYQVIPPKVQERNDTGAGDVFVGAFIAGLAMNMPITETLKVATGCSASAVMQQDSSSFDLEAAGKLKNQVSIIQL-

General information: TITO was launched using: RESULT: Template: 3Q1Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1707 1372 0.80 4.59 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 0.80 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_3Q1Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q1Y-query.scw PDB file : Tito_Scwrl_3Q1Y.pdb: