Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEEGLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALLMEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREKDGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQ-MVDKVIVAGRDGVQISTSKKGK 4M8L Chain:A ((10-221)) -DELKKLAATEAAKSITTEITLGVGTGSTVGFLIEELVNYRD---KIKTVVSSSEDSTRKLKALGFDVVDLNYAGEIDLYIDGADECNNHKELIKGGGAALTREKICVAAAKKFICIIDESKKVNTLGNFPLPIEVIPMARSYIARQIVKLGGQPVYRE----QTITDNGNVILDVYNLKIDNPLKLETELNQITGVVTNGIFALKPADTVIMATKDSNI--------

General information: TITO was launched using: RESULT: Template: 4M8L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1237 -94082 -76.06 -445.89 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -76.06 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_4M8L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M8L-query.scw PDB file : Tito_Scwrl_4M8L.pdb: