Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKIVKDILFLSQVSQPASQEDLY----LARDLQDTLLANRDTCVGLAANMIGVQKRVIIFNLG-------LVP-VVMFNPVLLSFEGSYE-AEEGCLSLVGVRST-KRYETIRLAYRDSKWQEQTITLTGFPAQICQHELDHLEGRII 5I2B Chain:A ((21-158)) -----------LLEVAKPVAQFDTPELHEIVADMFETM--HHANGAGLAAPQIGIGLQIIIFGF-NRYPDAPPVPETVLINPKLEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGYDQFGAKIDRVAEGFHARVVQHEYDHLIGKL-

General information: TITO was launched using: RESULT: Template: 5I2B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 473 -6482 -13.70 -53.57 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -13.70 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_5I2B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I2B-query.scw PDB file : Tito_Scwrl_5I2B.pdb: