Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVGENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKE----EFPARSAVEVARLPRDVKVEIEVIAEIG 3VCZ Chain:B ((26-152)) -TKVLHTDSAPAAIGPYIQGVDLGNMVLTSGQIPVNPATGEV-PADIAAQARQSLDNVKAVVEASGLTVGDIVKMTVFVKDLNDFGTVNEVYGNFFDEHNVAHYPARSCVEVARLPKDVGIEIEAIAVR-

General information: TITO was launched using: RESULT: Template: 3VCZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 584 -45087 -77.20 -366.56 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -77.20 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_3VCZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VCZ-query.scw PDB file : Tito_Scwrl_3VCZ.pdb: