Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQQAKSRKPQQPEYIFQVETLSHEGRGIAHYGSHPDHPADKHGKKVFIRYALPGETVKAQITHEAKRLEEAEMVALLAEPSANRVEAVCPHYGICGGCSMQHIHPDEQIRLKQNVLQSHLQHFAGIQPEQWLEPIRSLQSDYRRRARIGVRYLPKQDRLILGFREHHSNRLTSIHTCSVLDKKLSDSLPELRNLLQSLKGKVHIGHVELAKGDHETSLLVRHIEKLNNADVNQLRQFALHKGWQLYLQPKGPESLRRIDEEQGAMRLHYALNAFDVNFAFSPLDFTQVNATVNEQMVQLACELLQLQQGERVLDLFCGLGNFSLPLARCVGAKGVIVGVEASEEMVQRATDNVKRNNL---VQASFFSQDLTK-DFSHHSWANQGFDALLIDPPRAGAYEIIQYVPNFGAK-RIVYVSCNPATLARD----AGV-LVQHG--YQLKKAAVMDMFTHTEHVESIALFEKIQEIND 5HJM Chain:A ((170-346)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KENGVLIKVDLSKVFF-NPRMKGERY-RIAQLV--NDGERILVPFAGVIPYPLVIARFK--NVEVYAVEINEFAVKLAEENLELNRDRLKGKIKIIHGDVFEVLPN-LP----NFDRVVSPTPKG-VDALSLTLS--KAEKFLHYYDFVHESEIERFRERVLEECRRQGKECRVSVRKVSDYKPHVYKVCA------------

General information: TITO was launched using: RESULT: Template: 5HJM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 821 -34863 -42.46 -211.29 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -42.46 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_5HJM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HJM-query.scw PDB file : Tito_Scwrl_5HJM.pdb: