Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSLSQVNADVLKQAQQRIQQDLLTTLAAFSIPEPLKSAVHHAVMLGGKRVRPALCYATASLQDNPNFAAARRAAVAVELIHCYSLAHDDLPCMDNDLLRRGQPTCHVAFGEDTALLAGDILQSMAFEVLGSRLFDEQGQGTDAAIVLKQIQILAT--ASSKMVCGQVLDLQAEAKQISQDELENIHRNKTGALIQAAIMMGAVTIFPG-TDQAIPKLRQYGQAIGLAFQVQDDILDITSSTETLGKTAGKDEQVQKSTYPALMGLEQAQIYAKELHDQAFEALAHFGENAKELVEISQFLLARTN 4F62 Chain:B ((3-292)) -----MNLKQFSTYTQSRVDQYLEQQLSDYAPANQLHNAMRYSLF-GGKRIRPMLTYASAQLV-GDISSLTDASAAALESIHAYSLIHDDLPAM---------------FDEATAILAGDALQTFAFELLSNPTSA------QPELAIKLIQELVVASGRNGMITGQMIDLSSE--NISLAELEQMHVHKTGALIKASVRMGALS-TGQVKPEQLAKLDAYAHAIGLAFQVQDDIIDL------------------KATYPKLLGLDGAKALVVRLHEQAIAQISEFGDKSQPLTDLANYIID---

General information: TITO was launched using: RESULT: Template: 4F62.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1180 -94663 -80.22 -377.14 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -80.22 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_4F62.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F62-query.scw PDB file : Tito_Scwrl_4F62.pdb: