Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYHGME-----QPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKE-KNVWVVSQNIDGLHAKAKS--PQLVNFHGSLYQCHCRKCQQTVDWKEYL------HSDKHQAC------------------GGQIRPDIVLYGEGFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEPYTFIQEDGAVVFKKIQELGADYD 2HJH Chain:A ((33-316)) --FFTIDHFIQKLHTARKILVLTGAGVSTSLGIPDFRSSEGFYSKIKHLGLDDPQDVFNYNIFMHDPSVFYNIANMVLPPEKIYSPLHSFIKMLQMKGKLLRNYTQNIDNLESYAGISTDKLVQCHGSFATATCVTCHWNLPGERIFNKIRNLELPLCPYCYKKRREYFPERPPYILNSYGVLKPDITFFGEALPNKFHKSIREDILECDLLICIGTSLKVAPVSEIVNMVPSHVPQVLINRDPVKHAEFDLSLLGYCDDIAAMVAQKC----

General information: TITO was launched using: RESULT: Template: 2HJH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1113 -24839 -22.32 -105.70 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -22.32 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_2HJH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HJH-query.scw PDB file : Tito_Scwrl_2HJH.pdb: