Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERFDVERMIQMKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQ--TLVKKENVSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSRYLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVT-------S--IQLTKPNEKASKTITRSTVAAVLAALVEET-DISRTTLVLSEGSKELNTAFQEWAKEE 5F5N Chain:B ((1-220)) ----------NRTILVTGATGTQGGATVRALLARGRPVRALVRDPGTDAARALAAAGVSLVTGDLN-DQASLRAAMADVHGVFSVQTFMTPGGLGAELRQGRAVADAAAATGVRHVVYSSVGGADRA-----SGVPHFETKWTIERHLRSLGVPTTVLRPTFFMDNFAAWGPQAVDGTLVVRLPLKPQTRVQLIAAEDIGVFAATAFDDPDTYVGAALELAGDELTGPELAARFGE--

General information: TITO was launched using: RESULT: Template: 5F5N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1075 -49423 -45.97 -237.61 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -45.97 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_5F5N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F5N-query.scw PDB file : Tito_Scwrl_5F5N.pdb: