Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILEIMMKVKNIQSETAMKQHSESVVSNQFEPTCHSEHSSVSDVCSSSAFGLATRSSRARMKRASGFTLIEVMVVIVILGVLAALIVPNVMGRSEKAKIDTTQITLKGVAGALDQYKLDNGHFPTMQEGGLDALINQPA--TA-KNWMPGGYVKGGYPKDSWKNDIQYVVPGKDDHPFDLYSFGADGKEGGEGNDADIYYQP 3GN9 Chain:C ((5-110)) --------------------------------------------------------------------------------------------NKEKADQQKAITDIVALENALDMYKLDNSVYPTTD-QGLEALVTKPSSPEPRNYRN-GGYIKR-LPKDPWGNEYQYMSPGDK-GTIDIFTLGADGQEGGEGAAADIGNWN

General information: TITO was launched using: RESULT: Template: 3GN9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 405 21892 54.05 212.54 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : 54.05 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_3GN9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GN9-query.scw PDB file : Tito_Scwrl_3GN9.pdb: