Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIGTQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPLHAVDFLADYFKEK-TNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCSPVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMN---PEEGEYLKAITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEG-FKSFTAVETQGI-SRGQTLVDRYEF---WQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG 3T8J Chain:A ((1-310)) MRHFIIDCDTAEDDVLSLYLLLK-NNIDVVAVTIVEGNISYEQEVKNALWALEQVNR-EIPVYPGANKPLLKNYITVEKVHGKGGIGDVTVEPK-RLKAQEKHAALAIIDLANEYAGELEFLAISPLTNLALAYLLDNSIVKKIKKVWVMGGAVFGIGNITPVAEFNIWVDPDAAKIVFNAG-FDITMIPWDVIINYPVTDE---EWNVIKNMKTRMSELYVSMYLHYRQYSSTVQKINGHPHPDAITTAIAIDGSIATRREKRFVVIDNTDNITRGMTLVDRFDADTSWSDKPNAEIVYEINKKSFMEKIYDLLNW----------------

General information: TITO was launched using: RESULT: Template: 3T8J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1855 -80572 -43.43 -267.68 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -43.43 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.405

(partial model without unconserved sides chains): PDB file : Tito_3T8J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T8J-query.scw PDB file : Tito_Scwrl_3T8J.pdb: