Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSKANHAKTVICGIINVTPDSFSDGGQFFALEQALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDTWKSQVAEAALAAGADLVNDITGLMGDEKMPHVVAEARAQVVIMFNPVMARPQHPSSLIFPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK 1EYE Chain:A ((5-274)) --------PVQVMGVLNVTDDSFSDGGCYLDLDDAVKHGLAMAAAGAGIVDVGG--------------ETSRVIPVVKELAA-QGITVSIDTMRADVARAALQNGAQMVNDVSGGRADPAMGPLLAEADVPWVLMHWRA--VSADT---P---H---------VPVRYG-NVV-AEVRADLLASVADAVAAGVDPARLVLDPGLGFAKTAQHNWAILHALPELVATGIPVLVGASRKRFLGALLAGPD-GVMR----PTDGRDTATAVISALAALHGAWGVRVHDVRASVDAIKVVEAWMGAE-----------

General information: TITO was launched using: RESULT: Template: 1EYE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1458 -172174 -118.09 -672.55 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -118.09 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.619

(partial model without unconserved sides chains): PDB file : Tito_1EYE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EYE-query.scw PDB file : Tito_Scwrl_1EYE.pdb: