Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIE-NDQLVGLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLMLKNKIGILPVVD-NHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSLIVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIVRTSAKVL 2P9M Chain:C ((9-135)) -KVKDVMTKNVITAKRHEGVVEAFEKMLKYKISSLPVIDDENKVIGIVTTTDIGYNLIRDK----------YTLETTIGDVMTKDVITIHEDASILEAIKKMDISGINQLPVVDKNNKLVGIISDGDIIRTIS---------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2P9M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 529 -40734 -77.00 -339.45 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -77.00 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_2P9M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P9M-query.scw PDB file : Tito_Scwrl_2P9M.pdb: