Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYVRRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLGGPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEAMYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAGRGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVILTSQGEEGTY 2B3H Chain:A ((73-322)) -IKLLSSEDIEGMRLVCRLAREVLDVAAGMIKPGVTTEEIDHAVHLACIARNCYPSPLNY----YNFPKSCCTSVNEVICHGIPDRRPLQEGDIVNVDITL---------------------------YRNGYHGDLNETFFVGEVDDGARKLVQTTYECLMQAIDAVKPGVRYRELGNIIQKHAQANGFSVVRSYCGHGIHKLFHTAPNVPHYAKNKAVGVMKSGHVFTIEPMICEGGW-QDETWPDGWTAVTRDGKRSAQFEHTLLVTDTGCEILTRRLDS---

General information: TITO was launched using: RESULT: Template: 2B3H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1496 -156996 -104.94 -627.98 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -104.94 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_2B3H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B3H-query.scw PDB file : Tito_Scwrl_2B3H.pdb: