Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKIVKDILFLSQVSQPASQEDLY----LARDLQDTLLANRDTCVGLAANMIGVQKRVIIFNLG---------LVP-VVMFNPVLLSFEGSYE-AEEGCLSLVGVRST-KRYETIRLAYRDSKWQEQTITLTGFPAQICQHELDHLEGRII 5HGW Chain:B ((21-158)) -----------LLEVAKPVAQFDTPELHEIVADMFETM--HHANGAGLAAPQIGIGLQIIIFGFGSNNRYPDAPPVPETVLINPKLEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGYDQFGAKIDRVAEGFHARVVQHEYDHLIGKL-

General information: TITO was launched using: RESULT: Template: 5HGW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 478 -18309 -38.30 -150.07 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -38.30 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_5HGW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HGW-query.scw PDB file : Tito_Scwrl_5HGW.pdb: