Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAAS-GL-ETGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAP--HIPKPDPMLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEG-----LVGGGVYL-DFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLTDQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYFDTGLGDGRMVFVLPRE-NKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNITIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVESAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMGDEALWSAWLTSSKQNLTVALN 3AD8 Chain:B ((17-334)) ---------------PKKSYDVVIVGGGGHGLATAYYLAKNHGITNVAVLEKGWLAGGNMARNTTIIRSNYLWDE--SAGIYEKSLKLWEELPEELEYDFLFSQRGVLNLAHTLGD-------VR---ESIRRVEANKFNGVDAEWLTPEQVKEVCPIINTGDNIRYPVMGATYQPRAGIAKHDHVAWAFARKANEMGVDIIQNCEVTGFLKD-GEKVTGVKTTR---G---TILAGKVALAGAGHSSVLAELAG---FELPIQSHPLQALVSELFEPVHPTVVMS-----NHIHVYVSQAHKGELVMGAGIDSYNG--YGQRGAFHVIEEQMAAAVELFPIF--ARAHVLRTWGGIVDTTMDA------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3AD8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1636 9925 6.07 32.33 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 6.07 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_3AD8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3AD8-query.scw PDB file : Tito_Scwrl_3AD8.pdb: