Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSELSLAYIFHHQQLLVDQNLQLPKVEKLASDLLFTHDEQVIARDLLSEEAIPEGLQLVPIRQLISNWSKEQFLQASRAVQLLEWRRNHKFCSHCGHATEVHPTEYAMVCPSCRYHQYPRVNPCIITIITKGDDEILLAKSIHN-KTNMYGLIAGFVEVGETLEEAVQREALEEVGLKLKN--IQYMSSQPWPF----------PSNLMIAFRAEYESGEISLQEDEIADAQFFKFDQLPEIPFKGSIAHAMITQITQAK 1RYA Chain:A ((10-139)) -----------------------------------------------------------------------------------------------------------------ATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGYWFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHYVVLGFRFRVSEEELLLPDEQHDDYRWLTSDALLASD-----------------

General information: TITO was launched using: RESULT: Template: 1RYA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 494 -12524 -25.35 -107.04 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -25.35 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_1RYA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RYA-query.scw PDB file : Tito_Scwrl_1RYA.pdb: