Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------MPVVHAFRASQSGLDAGAMKRSSDSHSPPFF---FLSSACLAYRTSPMPSPRFARPEL-NGI-----------------------------------------------------------------ITVRRFQRPQLETEAPIRKGDLKEPLEAVAGRVGLRARCSTSAPSADLCLVDDHELVLMGKLVDKGVYYITGGVCIDAEGSDTSSQ---SPTAALVSSTACGAAPQ------------PPDITVGWCFDARMLLH----------------RSDMNRSPETPHRLQRAIETLQG-------------CERALDVLPVELL---APFTVHKDSSSG---------SDLGRAVPPISAANRSQWIPARLATYDEVCSFQDPQVYEHFLKSG---AALVNLKSDVYCN---DETSSVAVRLSAGAVIDASVAALRGVAASRSGTASSAAGRVHPLVSFCLVRPPGHHCTASQPSGFCLVNNVAIAVQQLRIRHASALPSGPPRIAILDLDVHFGEGTASFVEGACDPASLLYLSLHRYDKRQFYPFDGRGDTAYVGGSRHAASKGSICNVAVHTNGQQPARCEQVISDHLMNSVLEEIFLPRLAEFGPDLVMVSLGFDAAYGDPLGKMAV-EGGFASVLSRLKWWCLHDGRTAGLVVVLEGGYNPEAVAQGVLSVALALSLPRTDPLLQRFLEEKSPKVWSDLRQRQERRHREWQQLREERAEEDVGAAPSGLGSELKPAASDEPEQVQEDALLLDRHKRWCAALVAKVQQIHREAMTRER 5G0J Chain:A ((17-772)) VQGKSKATGTGLVYVDAFTRFHCLWDASHPECPARVSTVMEMLETEGLLGRCVQVEARAVTEDELLLVHTKEYVELMKSTQN-MTEEELKTLAEKYDSVYLHPGFFSSACLSVGSVLQLVDKVMTSQLRNGFSINRPPGHHAQADKMNGFCMFNNLAIAARYAQKRHRVQRVLIVDWDVHHGQGIQYIFEEDPSVLYFSVHRYEDGSFWPHLKESDSSSVGSGAGQGYNINLPWN-KVGMESGDYITAFQQLLLPVAYEFQPQLVLVAAG--FDAVIGDPKGGMQVSPECFSILTHMLKGVAQGRLVLALEGGYNLQSTAEGVCASMRSLLGDPCPHLPSSGAPCESALKSISKTISDLYPFWKSLQTFEGGPLSEVSPLPAPVCAEVKVSSPITGLVYDQRMMLHHNMWDSHHPELPQRISRIFSRHEELRLLSRCHRIPARLATEEELALCHSSKHISIIKSSEHMKPRDLNRLGDEYNSIFISNESYTCALLAAGSCFNSAQAIL--------------TGQVRNAVA--IVRPPGHHAEKDTACGFCFFNTAALTA-----RYAQSITRESLRVLIVDWDVHHGNGTQHIFE---EDDSVLYISLHRYEDGAFFPNSEDANYDKVG-----LGKGRGYNVNIPWNGGK-------MGDPEYMAAFHHLVMPIAREFAPELVLVSAGFDAARGDPLGGFQVTPEGYAHLTHQLMSLA--AGR---VLIILEGGYNLTSISESMSMCTSML----------------------------------------------LGDSPPSLDH-LTPLKTSATVSINN---VLRAHAPFWSSLRVNIPESLRLSL----

General information: TITO was launched using: RESULT: Template: 5G0J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3167 17786 5.62 32.10 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 5.62 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.315

(partial model without unconserved sides chains): PDB file : Tito_5G0J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G0J-query.scw PDB file : Tito_Scwrl_5G0J.pdb: