Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLTLVRHGE-AAPPVN--------GNDIKRPLTARGHAQAEQTATFLKDIV-KPNIFVVSPLLRAQETLAHIQTYFKDVPVLLCDK--------------------------------I------KPDDDAKE-----AIEWLSQI---------PYESIVVVCHMNVVGHIAELLTHET-----------FNPFALAEARIYDQAVIAN----GLSTQKNSFIPTI 2YN2 Chain:A ((13-238)) ENIYIARHGYRSNWLPKGPYPPPPTGIDNDVPLSEHGVEQAHELANYISKLDVKPEMIFSSPFYRCLETSKPTVEALK-IPLYVDRGVGEWYKPDRPIIPEPATHEVMSKFFPSMISPDWEPSIIPSNKGETEEDIFERCHKFWPVFIDRVERKFPNVKTIMIVTHAATKSALGMNLLKFSSAKEPIDNKGTFIRNGSCAIDKFEL-----PFEEREWKLTMNG----

General information: TITO was launched using: RESULT: Template: 2YN2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 602 -3686 -6.12 -26.14 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -6.12 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_2YN2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YN2-query.scw PDB file : Tito_Scwrl_2YN2.pdb: