Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLVVFSGAGMSAESGISTFRDSNGLWENYDIQQVATPEAWERNPALVQRFYNERRKNILEAQPNEAHQYIAKLQD-HYDVQVITQNIDDPHERAGSQNVLHLHGNIRLAKSSGPDAQYTTQFYEVNGWKLDLEQDFCP-NGYPLRPHVVWFGEAVPAYE--EAIRLVQSADIFIVIGSTLSVYPVAALVHEIPH-YSKAYYIAPQADHSRVPPQYKLLNMTATEGMHELFNQLTS 1M2N Chain:A ((12-241)) SKYLVALTGAGVSAESGIPTFRGKDGLWNRYRPEELANPQAFAKDPEKVWKWYAWRMEKVFNAQPNKAHQAFAELERLGVLKCLITQNVDGLHERAGSRNVIHLHGSLRVVRCTSCNNS-----FEVESAPKIPPLPKCDKCGSLLRPGVVWAGEMLPPDVLDAAMREVERADVIIVAGTSAVVQPAASLPLIVKQRGGAIIEINPDETPLTP-IADYSLRGKAGEVMDELVRHVR-

General information: TITO was launched using: RESULT: Template: 1M2N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1230 -20764 -16.88 -92.28 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -16.88 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_1M2N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M2N-query.scw PDB file : Tito_Scwrl_1M2N.pdb: