Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQAKKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWR-----------SLKRKINKKS-RPDMPESF---SEK--LNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN 4A82 Chain:B ((368-548)) ETVAFVGMSGGGKSTLINLIPRFYDV--TSGQILIDGHNIKDFLTGSLRNQI-GLVQQ-DNILFSDTVKENILLGRPTATDEEVVEAAKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEAT-------SALDLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVI----

General information: TITO was launched using: RESULT: Template: 4A82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 726 -4604 -6.34 -28.07 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -6.34 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.181

(partial model without unconserved sides chains): PDB file : Tito_4A82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A82-query.scw PDB file : Tito_Scwrl_4A82.pdb: