Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLTLVRHGEAAPPVN---GNDIKRPLTARGHAQAEQTATFLKDIVKPNIFVVSPLLRAQETLAHIQTYFKDVPVLLCDKI-----------------------------------KPDDDAKE--------AIEWLSQI-PYESIVVVCHMNVVGHIAELLTHET-----FNPFALAEARIYDQAVIANGLSTQKNSFIPTI 4IJ5 Chain:A ((2-194)) VKLILVRHAESEWNPVGRYQGLLDPDLSERGKKQAKLLAQELSREH-LDVIYSSPLKRTYLTALEIAEAKN-LEVIKEDRIIEIDHGMWSGMLVEEVMEKYPEDFRRWVEEPHKVEFQGGESLASVYNRVKGFLEEVRKRHWNQTVVVVSHTVPMRAMYCALLGVDLSKFWSFGCDNASYSVIHMEER---RNVILKL-----

General information: TITO was launched using: RESULT: Template: 4IJ5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 562 18321 32.60 129.93 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 32.60 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_4IJ5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IJ5-query.scw PDB file : Tito_Scwrl_4IJ5.pdb: