Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPE-KMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYL--FVVFCTPVGAYFKGGL--KPTNFIVSDYDRAAPKGT-GAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEVGAANFFGITKDGKFITPQ-SPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIEVVE 1I1L Chain:B ((5-307)) -----------------------------------KADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYDSHKGPV-VFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKN--------NLTSAYIRPLIFVGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAKAGGNYLSSLLVGSEARRHGYQEGIALD--VNGYISEGAGENLFEV-KDGVLFTPPFTSSALPGITRDAIIKLAKE-LGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSV---DGIQV--GEGRCGPVTKRIQQAFFGLFTGETEDKWGWLDQV-

General information: TITO was launched using: RESULT: Template: 1I1L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1594 -30653 -19.23 -103.56 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -19.23 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_1I1L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I1L-query.scw PDB file : Tito_Scwrl_1I1L.pdb: