Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQEQIEKKKEEKQKAEKAALEKK 5U5B Chain:A ((2-29)) --------------------------------------EIAIKKEIEAIKKEIAEIKKEIAIKK

General information: TITO was launched using: RESULT: Template: 5U5B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1 382 382.00 14.69 target 2D structure prediction score : 0.96 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 382.00 2D Compatibility (Sec. Struct. Predict.) : 0.96 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 1.112

(partial model without unconserved sides chains): PDB file : Tito_5U5B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U5B-query.scw PDB file : Tito_Scwrl_5U5B.pdb: