Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATAVNCNICFIGGGNMAQALIGGLISRG----LPPTRITVSDPVEQIR---QLLQEKEV-HVTQDNTAAIQNADVVVLAVKPQVLANVLRPLKGLL-SDKLVISIIAGAEIKTISNLID----SERIVRVMPNTPALVQTGAHGIYATDVVGASDRELTSQILAATGLTIWVNSEAQIDAVTAVSGSGPAYFFYLMESMIRAGKNLGLDEKVATALTLQTALGAAQMAITSSNTPSELRKNVTSPNGTTQAALEVFDRAQISQNIQSALAVAQKRSQELAQELSDSAK 5UAW Chain:B ((18-297)) -LYFQSMSVGFIGAGQLAFALAKGFTAAGVLAAH---KIMASSPD-MDLATVSALRKMGVKLT-PHNKETVQHSDVLFLAVKPHIIPFILDEIGADIEDRHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVE-EDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQSMADQE--

General information: TITO was launched using: RESULT: Template: 5UAW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1202 -59011 -49.09 -221.01 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -49.09 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_5UAW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UAW-query.scw PDB file : Tito_Scwrl_5UAW.pdb: