Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKILILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQIDIVEPDEMLVEVCREYFPDFAAG-LDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPFGH---TEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVYQAHIPTSPAGYWL-FGF--ASKKYHPV---KDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVEDILEEEEGKK 3ANX Chain:B ((5-289)) --MYFFEHVTPYETLVRRMERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKRVLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDDARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYRLVKAHLNPGGVMGMQAGMILLTH-HRVHPVVHRTVREAFRYVRSYKNHIP----GFFLNFGFLLASDAFDPAAFSEGVIEARIRERNLALRHLTAPYLEAMFVLPKDLLEALEKE----

General information: TITO was launched using: RESULT: Template: 3ANX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1616 -105692 -65.40 -384.33 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -65.40 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_3ANX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ANX-query.scw PDB file : Tito_Scwrl_3ANX.pdb: