Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYVRRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLGGPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEAMYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAGRGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVILTSQGEEGTY 4FUK Chain:B ((60-310)) HDDSKKTAEIQRIKTVCQLSREVLDIATAAAKPGITTDELDRIVHEATVERNMYPSPLN----YYGFPKSVCTSVNEVICHGIPDSRELEEGDILNIDVSS---------------------------YLNGFHGDLNETVFIGRPDDDSVRLVHAAYECLCAGIGVVKPEALYKQVGDAIEACASQYQCSVVRTYTGHGVGHLFHTSPTVCHYANNKSLGMMRPGHVFTIEPMINLGTW-QDVTWPDKWTSTTKDGRRSAQFEHTMVVTNGGVEIFTDWVDG---

General information: TITO was launched using: RESULT: Template: 4FUK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1500 -132226 -88.15 -526.79 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -88.15 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_4FUK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FUK-query.scw PDB file : Tito_Scwrl_4FUK.pdb: