Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVINKFVLMEQAKKVLKNAYCPYSKFPVGAAILFKDGKVITGANIENVSFGVTNCAERSAIFYGASQGYRKGDILAIAVAGET-EDYLPPCNICRQVMVEFCEPDTLVFLLNGKGNILELRLEELVPYSFSSLEM 2FR5 Chain:D ((11-142)) -EPEHVQRLLLSSREAKKSAYCPYSRFPVGAALLTGDGRIFSGCNIENACYPLGVCAERTAIQKAISEGY-K-DFRAIAISSDLQEEFISPCGACRQVMREFGTD-WAVYMTKPDGTFVVRTVQELLPASFGPEDL

General information: TITO was launched using: RESULT: Template: 2FR5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 662 -53871 -81.38 -411.23 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.81 3D Compatibility (PKB) : -81.38 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_2FR5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FR5-query.scw PDB file : Tito_Scwrl_2FR5.pdb: