Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIMATYETFAAVYDAVMDD--SLYDKWTNFSLRHLPKTKE-RKKLLELACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPG--YSYHENAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAGFKSFKLYADFEDKEPTETSTRWFFVAQK 3PX2 Chain:D ((15-241)) -----SGEIAELYDLVHQGKGKDYHREAADLAALVRRHSPKAASLLDVACGTGMHLRHLADSFGTVEGLELSADMLAIARRRNPD----AVLHHGDMRDFSLGRRFSAVTCMFSSIGNLAGQAELDAALERFAAHVLPDGVVVVEPWWFPENFTPGYVAAGTVEAGGTTVTRVSHSSREGEATRIEVH--YLVAGPDRGITHHEESHRITLFTREQYERAFTAAGLSVEFMPGGPSGR---------------

General information: TITO was launched using: RESULT: Template: 3PX2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1132 -14561 -12.86 -65.59 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain D : 0.69 3D Compatibility (PKB) : -12.86 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.341

(partial model without unconserved sides chains): PDB file : Tito_3PX2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PX2-query.scw PDB file : Tito_Scwrl_3PX2.pdb: