Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLVVFSGAGMSAESGISTFRDSNGLWENYDIQQVATPEAWERNPALVQRFYNERRKNILEAQPNEAHQYIAKLQD-----HYDVQVITQNIDDPHERAGSQNVLHLHGNIRLAKSSGPDAQYTTQFYEVN---G------------WKLDLEQDFCP---NGYPLRPHVVWFGEAVPAYE--EAIRLVQSADIFIVIGSTLSVYPVAALVHEIP-HYSKAYYIAPQADHSRVPPQYKLLNMTATEGMHELFNQLTS 5BWL Chain:A ((38-290)) AKHIVIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRAIAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATN-RFRFHFQGPCGTTLPEALA----

General information: TITO was launched using: RESULT: Template: 5BWL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1159 -20737 -17.89 -91.35 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -17.89 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_5BWL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BWL-query.scw PDB file : Tito_Scwrl_5BWL.pdb: