Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDLVATAQHWVNEGARRLHLVDLNGAFAGTPIHKPVVEAIAKAQPELPIQIGGGIRSLETIEHYLEAGVTFVIIGTKAVQEPEFVEEACKSF-AGHIIVGIDAMNG----MVATDGWANVTDVKATDLAKRFADAGVSSIVYTDIARDGMMQGVNVEQTVNLAQYSGLPVIASGGVTNLDDVRNLKGQLGILGAITGRAIYEGTLNLREAQLLLDENRL 1KA9 Chain:F ((5-246)) KRIVPCLDVHAGRVVKGVN--FVNLR-DAGDPVEAARAYDEAGADELVFLDISATHEERAILLDVVARVAE-RVFIPLTVGGGVRSLEDARKLLLSGADKVSVNSAAVRRPELIRELADHFGAQAVVLAIDARWRGDFPEVHVAGGRVPTGLHAVEWAVKGVELGAGEILLTSMDRDGTKEGYDLRLTRMVAEAVGVPVIASGGAGRMEHFLEAF-QAGAEAALAASVFHFGEIPIPKLKRYLAEKG-

General information: TITO was launched using: RESULT: Template: 1KA9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1381 -87343 -63.25 -368.54 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain F : 0.74 3D Compatibility (PKB) : -63.25 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.667

(partial model without unconserved sides chains): PDB file : Tito_1KA9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KA9-query.scw PDB file : Tito_Scwrl_1KA9.pdb: