Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILITGANGQLGTELRYLLDERNE-EYVAVD-VA------EMDITDAEMVEKVFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTDYVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGK---NFVFTMQNLAK-THKTLTVVNDQYGRPTWTRTLAEFMTYLAENR----KEFGYYHLSNDATEDTTWYDFAVEILKDTDVE---VKPVDSSQFPAKAKRPLNSTMSLAKAKATGF-VIPTWQDALQEFYKQEVR 2YDX Chain:E ((4-298)) RVLVTGATGLLGRAVHKEFQQN-NWHAVGCGFRRARPKFEQVNLLDSNAVHHIIHDFQPHVIVHCAAERRPDVVEN-QPDAASQLNVDASGNLAKEAAAVGAFLIYISSDYVFDGTN---PPYREEDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYGEVEKLEESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIKGTFHWSGNE--QMTKYEMACAIADAFNLPSSHLRPITDSPV-LGAQRPRNAQLDCSKLETLGIGQRTPFRIGIKESLWPFLI

General information: TITO was launched using: RESULT: Template: 2YDX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1522 30769 20.22 111.89 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain E : 0.72 3D Compatibility (PKB) : 20.22 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_2YDX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YDX-query.scw PDB file : Tito_Scwrl_2YDX.pdb: