Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFQRMTDLNLAGKRVLIREDLNVPVKNGVITSDARLRAALPTIKAALEKGAAVMVFSHLGRPVEGEPKPEQSLAPVAAYLTEALGQEVKLFTDYLDG------VEVEAGQVVLLENVRFNPGEKKNN-------PELAQKYAALCDVFVMDAFGTAHRAEASTEGVARFAPVAAAGPLLAAELDALGRAMQTPEKPMVAIVAGSKVSTKLDVLNSLSG--ICDQLIVGGGIANTFLAAAGYNVGKSLYEA-------DLVETAKQIAAK--VSVPLPTDVVVADASQINFEDFLGSLAAAQAVIK-KVEDVTANDMILDVGPETAKAFANILTTSKTILWNGPVGVFEVDQFGEGTKALSLAVAQSDAFSIAGGGDTLAAIDKYNVADQIGYISTGGGAFLEFVEGKTLPAVAVLLERA 2CUN Chain:A ((3-401)) ----RLEDFNFHNKTVFLRVDLNSPMKDGKIISDARFKAVLPTIRYLIESGAKVVIGTHQGKPYSED---YTTTEEHARVLSELLDQHVEYIEDIFGRYAREKIKELKSGEVAILENLRFSAEEVKNKPIEECEKTFLVKKLSKVIDYVVNDAFATAHRSQPSLVGFARIKP-MIMGFLMEKEIEALMRAYYSKDSPKIYVLGGAKVEDSLKVVENVLRRERADLVLTGGLVANVFTLAKGFDLGRKNVEFMKKKGLLDYVKHAEEILDEFYPYIRTPVDFAVDYK----G-ER------VEIDLLSENRGLLHQYQIMDIGKRTAEKYREILMKARIIVANGPMGVFEREEFAIGTVEVFKAIADSPAFSVLGGGHSIASIQKYGIT-GITHISTGGGAMLSFFAGEELPVLRALQIS-

General information: TITO was launched using: RESULT: Template: 2CUN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2169 -38263 -17.64 -102.31 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -17.64 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_2CUN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CUN-query.scw PDB file : Tito_Scwrl_2CUN.pdb: