Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDITLAPVQPDQPTHLMATYGRQAISFVRGRGAYLYTQDGTEYLDALTGIAVCGLGHTHPTIAEAIAE----QAATLVHTSNLFEIPWQTAAAQKLAEV------SGMEEIFFSNSGAESNEGAIKIARKFGTQQGISLPKIIVAEQSFHGRTLATLSATGNKKVQDGFAPLVEGFIRVPFGDIEAIQEAALQHPDIVAILIEPIQGEGGINTAPQGFSYLEEVRQICNQHNWLMMLDEIQTGNGRTGKYFAYQHTNIIPDVLTTAKGLGNGF-PVGAVMTQG----KAVGLLGPG-SHGSTYGGTVLGSRVVYTVIDTIQKENAVENAAVVGNYIVDQLRAQLSDKN---VQVRGFGMMIGIQ----LPKD------------------------------CAELVAIARDEYKLIINVTAGS--VVRLLPPINMTQAQADILLECLVALITNYL 4PPM Chain:A ((419-835)) ---------------------------FRSAAGTQLYDDAGEAFLDMVAGYGCLNLGHNPQPVVNALKNYLDAQGPNFIQ---YISIPEQTA---KLAEVLCRLAPGNMGRVFFSNSGTEAVEAAMKIAKASTGKPGIAYLR-----NSYHGKTLGALSITGRDKHRRYFTPLLDAMVEVPFGDLAALREA-LNREDVGALMIEPIQGEGGVHIPPAG--YLQAVQQLCRETGVLLMVDEVQTGLGRTGKLFACEWDGIEPDVLMLSKSLSGGLIPIGATLCRADLWQKAYGTADRFLVHSSTYGGGNLASVVALSALREILAQDLVGHAERMGAYF-KQALSEIAARYPFVSEVRGRGLMLGIQFDQA----HTTWKFLPDPVQAHLRAAMDRMEQALGEMFCMKFVTKLCQDHKILTFITANSSTVIRIQPPLIISKAEID--------------

General information: TITO was launched using: RESULT: Template: 4PPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1855 10618 5.72 31.05 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 5.72 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_4PPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PPM-query.scw PDB file : Tito_Scwrl_4PPM.pdb: