Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLSCIQQPHQHLQANLEGGVLTLAINRPEAKNALYGELYLWIAKALDEADQNKDVRAVVLRGAEHDFTAGNDMKDFMGFVQNPNAGPAGQVPPFVLLKSAARLSKPLIIAVKGVAIGIGVTILLQADLVFADNTALFQIPFVSLGLSPEGGASQLLVKQAGYHKATELLFTAKKFNAEAAVQAGLVNEIVE------DAYATAQHLTALPLASLKQTKALMKHDLDQIIECIDHE-TEIFMQRVQSPEMLEAVQAFMQKRKPDFSQFNGFVAQTF 4MOU Chain:C ((34-294)) ---------TGRISVAIADGVASVEISNLAQRNALTKDMCLEIQELMPQLDADPDVAVVVLRGAGDTFCAGASISELASVLLDPQPDGSTADHLSRADSAIAALSKPAVALVDGACMGGGWQIASACDFIIANERAVIGLTPAKIGIIYPRPGLERLVRLVGHANAKYILLTGQTFSAPEARALGLVADVVPSESFEEKCAALVRSLRSRSRFSMHSMKRLVDLTDS---AGIDQEWAAAWSAMPDSPDMGIGISAFLNREQPRFMWRPA------

General information: TITO was launched using: RESULT: Template: 4MOU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1229 -142251 -115.75 -566.74 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.72 3D Compatibility (PKB) : -115.75 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_4MOU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MOU-query.scw PDB file : Tito_Scwrl_4MOU.pdb: