Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALVKTKLAAAPQPSRTPTPGSSPKEWTPPTHTHESPEHLSTDEPDVLVPAHKYVYDVKTCAWKGVDTMLRVLHPNRGLSQGTMRVCFALEELDETGFSSRMVAKMFRHNISKVVESD-YFNEGEAQCICGIFAEKFNRVQVPAGFQRHIVSFLQCETVRIKLSEVPEAYQHKRSGFFSYRTTDSADILFTMEPRLAGNFTKYTNNFGDVYDGFERRQSLEEEKKRHRVLMAVEAFSHFTLVESGGSMLVCDLQGVNDFLTDPQIHTEDGKGLGMGNMGQDGIRKWMEAHVCNEVCRAIMLEPLSKGLRNFTRTAENESRVSYYQILRAKLRSQTPVRPEDIIPLSKPLSLMSDDERLEYAMRLSALLSE 4ZME Chain:A ((97-272)) ------------------------------------------------------------------------------------------EAMTQLKNGTKFVLKLYKK---QQASRELYFEDVKMQMVCRDWGNKFNQKKPPKK-----IEFLMSWVVEL-IDRSP--------------SSNGQPILCSIEPLLVGEFKKNNSNYGAVLTNRSTPQ----------------AFSHFTYELSNKQMIVVDIQGVDDLYT-PQIHTPDGKGFGLGNLGKAGINKFITTHKCNAVCALLDLD--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZME.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 774 -2896 -3.74 -16.94 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -3.74 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_4ZME.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZME-query.scw PDB file : Tito_Scwrl_4ZME.pdb: