Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIAQLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIASSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQSMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN 4RYF Chain:H ((3-193)) LIPTVI--------AYDIYSRLLKDRIIMLGSAIDDNVANSIVSQLLFLDAQDPEKDIFLYINSPGGSISAGMAIYDTMNFVKADVQTIGMGMAASMGSFLLTAGANGKRFALPNAEIMIHQPLGGAQG--QATEIEIAARHILKIKERMNTIMAEKTGQPYEVIARDTDRDNFMTAQEAKDYGLIDDIIINK---

General information: TITO was launched using: RESULT: Template: 4RYF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 844 -120656 -142.96 -659.32 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain H : 0.93 3D Compatibility (PKB) : -142.96 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_4RYF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RYF-query.scw PDB file : Tito_Scwrl_4RYF.pdb: