Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIALFYMGGTFGCI-GEPLAPMPYDQFLPQLEKVIPPHLTVDC----FAAPNIVDSSACTAPDWLRLIQRIQQLQLEGYQHFVVIHGTDTLSYAAATLARFLGQS-CHIVITGSQYPLLNIQGDNTREFTDAIENLYLALEQVIALLVGAYLAFHHQVFHAQTALKTHTTELDAFS-----------GLSSNVEFTPQQN--ELIVQDAQIEKAASFQLLNWMMQPIATQHLVQQLRHLLPAPPHFLVLQGFGTGNIAV-NDEFLATLDELYTRGCVPI-LATQVTFGGID-QRYAISTWAKTAKIVINDAHSHADLYAKALQIYLKYPTPEQWLNHWNENLH 4Q0M Chain:A ((1-286)) MMKILLIGMGGTIASVKGEN----GYEASL-SVKEVLDIAGIKDCEDCDFLDLKNVDSTLIQPEDWVDLAETLYK-NVKKYDGIIVTHGTDTLAYTSSMISFMLRNPPIPIVFTGSMIPA-------TEENSDAPLNLQTAIKFATSGIRGVYVAFNGKVMLGVRTSKVRTMSRDAFESINYPIIAELRGEDLVVNFIPKFNNGEVTLDLRHDPKVLVIKLIPGLSGDI--------FRAAVELGYRGIVIEGYGAGGIPYRGSDLLQTIEELSKE--IPIVMTTQAMYDGVDLTRYKVGRLALRAGVI------------------------------------

General information: TITO was launched using: RESULT: Template: 4Q0M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1418 -21957 -15.48 -83.17 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -15.48 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.356

(partial model without unconserved sides chains): PDB file : Tito_4Q0M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q0M-query.scw PDB file : Tito_Scwrl_4Q0M.pdb: