Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFEQKPKVTVILANLGTPDEATVPAVRRFLKQFLSDPRVIEIPKFIWWIILNLFVLPFRPKRAAHAYASVWSTDSPMREIVFEQTQRVQAYLERENKQFDLTVLPAMTYGNLGIDAVLEKLSAHPQEHVILLPLFPQYSATSTAPLYDAFAKWIPTQRNLPGL--TIIKDYYQHPMFIQALAESVLAYQEQHGKPEK-----LLMSFHGIPQPYADKGDPYADRCRITAKLVAEALHLKDDEWAISFQSRFGKQEWVKPYTDQLLQDWAKQGVKSVQVLSPAFSADCLETLEELAIQNAE-LFQQAGGGSYAYIPALNSDQAHIDLLAGLVQANLDALTHTLAHR 1HRK Chain:A ((107-327)) -------------------------------------------------------------------------------------------------------------------EEAIEEMERDGLERAIAFTQYPQYSCSTTGSSLNAIYRYYNQVGRKPTMKWSTIDRWPTHHLLIQCFADHILK-ELDHFPLEKRSEVVILFSAHSLPMSVVNRGDPYPQEVSATVQKVMERLEYCNP-YRLVWQSKVGPMPWLGPQTDESIKGLCERGRKNILLVPIAFTSDHIETLYELDIEYSQVLAKECGVENIRRAESLNGNPLFSKALADLVHSHIQS--------

General information: TITO was launched using: RESULT: Template: 1HRK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 978 -9622 -9.84 -45.17 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -9.84 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_1HRK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HRK-query.scw PDB file : Tito_Scwrl_1HRK.pdb: