Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIVKIENVRVLDPIQKTDSVQTVYLQDGKLVAPVDQVEQTIDGQGKWLMPTMVDLCARLREPGQQQHGTLKSEGKAARANGILHVITPPDSKPIVQDNGALIHGLIEKAWHDGGIHMHIIGAQTHGLNGKQPANMAGLKKGGCTAVSNANAAFENDDVVVRTLEYAAGLGLTVVFYAEEPQLAKDGCAHEGFIASRQGLPMIPAIAETVAIAKHLLMIEATGVKAHFGLLSCGASVELIRAAKAKGLPVTADVAMHQLHLTEQLIDGFNSLAHVRPPLRSAQDKELLRQGLKQGVIDAICTHHEPLSSSAKMAPFAETQPGITAFDTYVALGIQLINEGLFEPLEWVTKVTSAPAQVANMTARWQAEAGWVLVDPELSWTVSKDTILSQGKNTPLLGQKLTGKVLQTFAV 4C6B Chain:A ((11-340)) -------------------------------------------------LPGLIDVHVHLR-------EDFASGTAAALAGGITMVCAMPNTRPPIIDAPAL--ALAQKLAEAGA---------------------------RCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIV-AHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDD-------GEVRPELGSRQDVEALWENM--AVIDCFASDHAPHTLEEKC----RPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQ---EDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTV------

General information: TITO was launched using: RESULT: Template: 4C6B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1780 -182266 -102.40 -601.54 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -102.40 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.378

(partial model without unconserved sides chains): PDB file : Tito_4C6B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C6B-query.scw PDB file : Tito_Scwrl_4C6B.pdb: