Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTESASGTPTQPSLFVLVKQILILAGFWWIGYLLHQKLG--VPV-S-AGILGMFLLLLCLFFKIIKMDQVAMGATVVL-GELLLFFVPVV---VAVVQYKTLFMTEGWQIVLSIAVGTILVMLSTSLTIHYYNRLKAYLQARKRLQHKHI 5A1S Chain:C ((53-170)) -------------------GGFALMFVMGAIFGEIGKRLPIFNKYIGGAPVMIFLVAAYFVYAGIFTQKEIDAISNVMDKSNFLNLFIAVLITGAILSVNRKLLLKSLLGYIPTILAGIVGASLFGIVIGLC-------------------

General information: TITO was launched using: RESULT: Template: 5A1S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 173 -29682 -171.57 -282.68 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -171.57 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.65 QMean score : -0.134

(partial model without unconserved sides chains): PDB file : Tito_5A1S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5A1S-query.scw PDB file : Tito_Scwrl_5A1S.pdb: