Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMRRVLIAIASLVVITSIVFFTYQHIKQQQAKDSSQMFDVVMTEKMNQLYDQAQDWTKPVQLDIHDKRLAGHYKQVSEFLLSYWVQNVNAR-NEYLRELKAAKWDTFLNVDRLDHDKKQKYAETEKMLADVRRASDKYQSEYEKIHKTFLAKNQ------ELSVDKEMRQILEIKLGAQQKADQDHAIFMIELQILDKAEEMFKLLKTYPWQKKDQMILFHENAQVKKFNALYQDVLKLNAKMEKLRKRM 4ZKT Chain:A ((678-798)) -------------------------------------------------------------------------WKEVYSFIVSNWMTKINTQFNKRKEQMYQALQNQVNAIKTIIESKYNSYTLEEK--NELTNKYDIKQIENELNQKVSIAMNNIDRFLTESSISYLMKLINEVKINKLREYDENVKTYLL-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZKT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 190 10563 55.59 96.03 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 55.59 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.308

(partial model without unconserved sides chains): PDB file : Tito_4ZKT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZKT-query.scw PDB file : Tito_Scwrl_4ZKT.pdb: