Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLEKILQSQGFGSRKYCQQLIKNGSVSIDGEVVSDLKKQFSPENFEFSVFGQNYQYREKIYIALRKPQGFECSHHPQHHQSVFSLLPETMIHRGVQAVGRLDQDTTGLLLLTDDGKYLQVLTHPRKHVPKVYHVTTYDPVTPEQIEMLIQGVNLHQEKEVFAATDVALLGTHQLTMTIHQGVYHQVKRMIAAVGNKVEKLHRHQIGQLVLPEIEDGDWVYLSEQEKQLAQNII 2GML Chain:A ((3-163)) ------------------------------------------------------------VLIALNKPVGIVSTTEDGERDNIVDFVNHS---KRVFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITEEFIRGMSAGVPILGTVTKKCKVKKEAPFVFRITLV--QGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPLGEWRDLTDDE--------

General information: TITO was launched using: RESULT: Template: 2GML.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 730 -77829 -106.62 -483.41 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -106.62 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_2GML.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GML-query.scw PDB file : Tito_Scwrl_2GML.pdb: