Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLWLFEQLAGYHSSFQVVRYLTLRSLLSVLTSLTIGLVLGPIMIRKLQALKYGQAVSSFAPENHAKKMGTPTMGGILILLSIGISTLLWADL----SN--PYVWIVLGVMVVFGAVGWADDWIKIRYKDNAGLPARKKFFWTSVASLGAGIALYLIATQQSNAEYTANMLDLLIPFFKNLSIPLSIVPLGLAFIVFTYLVINGASNAVNLTDGLDGLAIMPVVMVATGLGVFAYLSGDIRFANYLHIPYVKYTSELVVICSAMIGAGLAFLWYNAHPAQVFMGDVGALALGAMLGTIAVMVRQEIVFAIMGGVFVMEAVSVFLQIGSLR--MRNKRV-FLM--APL------------HH-------------------------------------HY-EK-QGWKETQVVIRFWIITIMLVVLGLMTLKLR 5LEV Chain:A ((9-399)) -------------------PMPLLINLIVSLLGFVATVTLIPAFRGHFIAARLC-----GQDLNKTSRQQIPESQGVISGAVFLIILFCFIPFPFLPHHEFVALIGALLAICCMIFLGFADDVL--------NLRWRHKLLLPTAASLPLLMVYFT--N--------FGNTTIVV------HLDLGI-----LYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNLVELEGDC--------RDDHVFSLYFMIPFFFTTLGLLYHNWYPSRVFVGDTFCYFAGMTFAGVGILGHFSKTMLLFFMPQVFNFLYSLPQLLHIIPCPRHRIPRLNIKTGKLEMSYSKFKTKSLSFLGTFILKVAESLQLVTVHQSETEDGEFTECNNMTLINLLLKVLGPIHERNLTLLLLLLQILGSAITFSIR---

General information: TITO was launched using: RESULT: Template: 5LEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1417 -233319 -164.66 -757.53 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -164.66 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.304

(partial model without unconserved sides chains): PDB file : Tito_5LEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LEV-query.scw PDB file : Tito_Scwrl_5LEV.pdb: