Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYRLSMSLIANTTVVRGRFLDIQQTVSEPTDIPNQVRYLEDGVLISEHGKIKWFGAWEDAQQHLPAGVEVQHYPEQLIVPGFIDTHIHFPQTEMVG--AYGEQLLSWLNTYTFPTEIQFQDKTYASEIAQFFVQELLKHGTTTAL--VFCTVHPESVDALFEAAERVQMRLIAGKVLMDR--------------NAPEA-LCDTPE---TAYSNTKALIEKWHGKGRALYAITPRFAPTSTPEQLERAGQLKQEFPD---VYVHTHLSENKDEIAWVKSLFPEQAGYLDVYQHYGLTGKRSVFAHCVHLEDEEWQCMHDTQSAIAFCPTSNLFLGSGLFPLKKTWEKQVKVGLGTDIG-AGTSFSL---LQTVNEAYKVQQLQGDKLSAYEALYHATLGGAKALDLQDQLGNFNIGKEADFVVLNLKPTALQELRQTKSKSVEDSLFALFTLGDDRNIEATYIYGNRAYQKETAK 4V1X Chain:A ((39-418)) --------------------------------------RVLGDSWVHVQDGRIVALGV--HAESVPPPADRVIDARGKVVLPGFINAHTHVNQILLRGGPSHGRQFYDWLFNVVYPGQKAMRPEDVAVAV-RLYCAEAVRSGITTINENADSAIYPGNIEAAMAVYGEVGVRVVYARMFFDRMDGRIQGYVDALKARSPQVELCSIMEETAVAKDRITALSDQYHGTAGGRISVWP--APATTTAVTVEGMRWAQAFARDRAVMWTLHMAES-DHDERIHGMSP--AEYMECY---GLLDERLQVAHCVYFDRKDVRLLHRHNVKVASQVVSNAYLGSGVAPVPEMVERGMAVGIGTDNGNSNDSVNMIGDMKFMAHIHRAVHRDADVLTPEKILEMATIDGARSLGMDHEIGSIETGKRADLILLDLR------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4V1X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1877 -38523 -20.52 -109.75 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -20.52 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_4V1X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4V1X-query.scw PDB file : Tito_Scwrl_4V1X.pdb: